ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2017, Vol. 48 ›› Issue (2): 260-271.doi: 10.11843/j.issn.0366-6964.2017.02.009

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Effects of Supplementation in Cold Season on Morphological Development of Small Intestine and the Expression of Nutrient Transporter Gene

JING Xiao-ping1, PENG Quan-hui1, HU Rui1, LI Qian1, ZHOU Ting1, PEI Zhao-xi1, ZHAO Suo-nan2, YANG Tao2, MA Chao-yin2, WANG Zhi-sheng1*   

  1. (1. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China; 2. Haibei Demonstration Zone of Plateau Modern Ecological Husbandry Science and Technology, Haibei 810299, China)
  • Received:2016-06-16 Online:2017-02-23 Published:2017-02-23

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Abstract:

The objective of this study was to investigate the effect of supplement urea-molasses lick block or concentrate on morphological development of small intestine and the expression of nutrient transporter gene. Eighteen healthy, 1.5-year-old Tibetan sheep ewes ((29.4±1.79) kg) were selected and randomly assigned into control group (CON group), urea-molasses lick block supplementation group (BS group) or concentrate supplementation group (CS group). The CON group were provided with oat hay ad libitum, the BS group were provided with oat hay ad libitum and supplemented with lick block ad libitum, the CS group were provided with oat hay ad libitum and supplemented with concentrate (200 g/sheep/day), during 60 days feeding experiment period. All ewes were slaughtered after the feeding trial. Sampled each segment of the small intestine, and then, the morphological development and nutrient transporter gene expression of small intestine were measured through making sections of small intestine tissue and fluorescence quantitative PCR. The results showed as follows: (1) Supplementation with concentrate or lick block significantly increased the intake of digestible energy and crude protein of Tibetan sheep (P<0.05), and the CS group was significantly higher than the BS group (P<0.05); (2) Supplementation with concentrate or lick block significantly enhanced the villus width of duodenum, jejunum and ileum of Tibetan sheep (P<0.05), and the CS group was significantly higher than the BS group (P<0.05), and the villus height of duodenum and ileum were significantly enhanced and the crypt depth of jejunum and ileum were significantly reduced in the CS group compared with the CON group (P<0.05); (3) The relative expression of insulin-like growth factor binding protein 5 (IGFBP5) mRNA in both of the duodenum and jejunum mucosa were significantly enhanced in both of the CS and BS groups compared with the CON group (P<0.05), and the CS group was significantly higher than the BS group (P<0.05), and in the ileum mucosa the CS group was significantly higher than both of the BS and CON groups (P<0.05); (4) The CS group had the higher relative expression levels of cationic amino acid transporters 1 (CAT1), L-type amino acid transporter 1 (LAT1), peptides transporter 1 (pepT1), Na+-dependent glucose co-transporter 1 (SGLT1) and facilitative glucose transporter 2 (GLUT2) mRNA in duodenum mucosa compared with the BS and CON groups (P<0.05). The CAT1, pepT1 mRNA relative expression in jejunum mucosa and the CAT1, LAT1, SGLT1 and GLUT2 mRNA relative expression in the ileum mucosa in the BS group were significantly higher than both of the CS and CON groups (P<0.05). We concluded that supplementation with urea-molasses lick block or concentrate increased the intake of energy and protein of Tibetan sheep in cold season, and promoted the morphological development of small intestine, and the expression of transporter gene mRNA of amino acids, peptides and glucose were enhanced in duodenum mucosa by supplementation with concentrate and they were enhanced in both of the jejunum and ileum mucosa by supplementation with urea-molasses lick block.

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